Performing FRAP Studies with a Widefield Microscope

Presenter: Chris Baumann, Ph.D., Product Manager at MAG Biosystems
January 31, 2008
Thursday, 1:30 pm, Eastern (New York)

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Fluorescence Recovery After Photobleaching (FRAP) is a powerful method for measuring global protein diffusion and has opened new areas of study in molecular and cell biology. In this technique, molecules of interest are fluorescently labeled and a focused laser is used to rapidly and acutely inactivate the fluorescence by photobleaching a small subregion of the cell. Mobile molecules then repopulate the bleached area over time. The rate of recovery provides an accurate measure of the molecular mobility within the cell. Performance of accurate FRAP studies, however, requires paying special attention to preservation of specimen integrity and places special burden on the illumination, optics, and camera so that overall efficiency and speed is optimized.

Attendees at this free online seminar will learn how modern FRAP systems are constructed and utilized to effect accurate, high-speed studies of intracellular diffusion and discuss some of the desired properties of FRAP analysis software.

Bring your questions to this live, interactive web-based seminar.

Subjects include:

• Considerations when defining a system for FRAP studies
• 2D and 3D FRAP applications
• Widefield and confocal approaches
• FRAP-related studies such as photoactivation and photoconversion (iFRAP)

Provided at no cost, this webinar is sponsored by MAG, the Microimaging Applications Group. MAG is a group of imaging companies who work together to provide an unparalleled range of microimaging solutions to science and industry.

About the Presenter

Chris Baumann, Ph.D. is the MAG Biosystems Product Manager at Photometrics, providers of FRAP systems, spectral imaging systems, and whole-animal imaging systems. Chris received his Ph.D. from the University of Buffalo and following his postdoctoral work at the NIH, he managed a core imaging facility there. Chris has worked in the field of fluorescence imaging for nearly 10 years.