Paul French, Professor
Photonics Group, Physics Department, Imperial College London
The Photonics Group in the Physics Department at Imperial College London develops instrumentation for multidimensional fluorescence imaging – spanning a wide range of applications, from super-resolved microscopy through automated fluorescence lifetime imaging for high content assays to endoscopy and optical tomography.
The availability of Scientific CMOS cameras has been transformative for their research because the technology provides unprecedented imaging performance with high resolution and high frame rates. The team particularly uses Scientific CMOS cameras for localization and light sheet microscopy.
STORM [i, ii] for super-resolved microscopy is a particular interest and the team recently published an approach using low-cost diode lasers [iii]. “Our goal is to develop instruments that provide state-of-the-art performance while reducing the cost where possible so that more users are able to access such advanced imaging capabilities,” shared Professor Paul French.
The Prime 95B camera is specified to provide >95% quantum efficiency, giving us the advantages of Scientific CMOS [sCMOS] with fantastic sensitivity.
- i M. J. Rust, M. Bates, and X. Zhuang, Nat. Methods 3, 793–796 (2006).
- ii M. Heilemann, S. van de Linde, M. Schuttpelz, R. Kasper, B. Seefeldt, A. Mukherjee, P. Tinnefeld, and M. Sauer, Angew. Chem. Int. Ed. 47, 6172–6176 (2008).
- iii K. Kwakwa, A. Savell, T. Davies, I. Munro1 S. Parrinello, M.A. Purbhoo, C. Dunsby, M.A.A. Neil and P.M.W. French,J. Biophotonics 9 (2016) 948–95, DOI 10.1002/jbio.201500324