Fluorescent Speckle Microscopy

Visualizing fluorescent proteins, whether injected or expressed, in living cells continues to yield much information. Fluorescent speckle microscopy is a labeling strategy that uses low levels of fluorochrome such that the label is incorporated into the structure of interest in a non-homogeneous manner (i.e., the structure of interest is seen to be "speckled" with fluorochrome).

This approach has utility for studies exploring dynamic assembly, movement of structures, and turnover of proteins at the limits of optical resolution. The strategy also reduces out-of-focus fluorescence and greatly improves the visibility of fluorescently labeled structures in thicker regions of living cells. Due to the low amount of fluorescent signal and the fast dynamic events usually being imaged in speckle microscopy, selecting a high-sensitivity, low-noise camera is of paramount importance. Photometrics EMCCD cameras are engineered with the industry's most innovative electronics to enable excellent detection and quantitation capabilities.

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