Sequential Color Imaging

Sequential color acquisition is often used to produce high-spatial-resolution, true-color images. High-performance CCD cameras are utilized in conjunction with either color-separation filters or liquid-crystal tunable filters to generate individual red, green, and blue images that are combined in software to produce a true-color image.

In sequential acquisition, each image (red, green, blue) uses the entire CCD array. Every pixel can be utilized to produce fine detail or a large field of view. Additionally, sequential acquisition allows independent variations in exposure time for each color band. Unlike single- or three-chip color cameras, sequential acquisition overcomes the common problem of blue sensitivity by allowing the camera to integrate longer while acquiring the blue image, effectively enhancing the quality of the final composite image.

Sequential Color image
Image courtesy of Photometrics.

Tissue sections are often viewed using a microscope and sequential color acquisition. Three color-band images, as well as a final composite color image, are shown above. Each individual color-band image was acquired using a filter wheel on a Photometrics CCD camera.

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