Fluorescence Correlation Spectroscopy, University of California, Irvine
Fluorescence Correlation Spectroscopy
Prime 95B Scientific CMOS
Image courtesy of
Prof. Enrico Gratton, Founder and Principle Investigator
Laboratory of Fluorescence Dynamics
University of California, Irvine

Arabidopsis in agar expressing GFP, imaged using SPIM with the Prime 95B uncorrected for camera noise. Pair correlation function showing obstacles to diffusion due to the cell walls in regions of strong anisotropic motion.

The parallel lines of signal clearly define the boundaries of diffusion at each side of the cell walls. Unlike super-resolution microscopy or other methods, fluctuation correlation spectroscopy images the diffusion itself, rather than the cellular structures which are often not visible.

Time series of 2100 frames over 10.5s, 256×256 pixels, 140nm effective pixel size.