Single Molecule TIRF Microscopy
Prime 95B Scientific CMOS Camera
Image courtesy of
Aleks Ponjavic, Postdoctoral Researcher,
Klenerman Group, University of Cambridge

Jurkat T-cells bound to fibronectin. CD45 molecules on the cell surface labelled with Alexa 488.

A. Maximum intensity projection of 500 frame 33 ms exposures taken with the Evolve Delta EMCCD camera using 250x EM gain.
B. Maximum intensity projection of 500 frame 33 ms exposures taken with the Prime 95B.
C. Maximum intensity projection of 500 frame 33 ms exposures taken with the Prime 95B binned 2x2.