Structured Illumination Microscopy (SIM)
Structured Illumination Microscopy (SIM)
Prime 95B Scientific CMOS Camera
Image courtesy of
Guy Hagen, PhD, Research Associate,
University of Colorado, Colorado Springs

Cross section of a rabbit seminiferous tubule acquired using Dr. Hagen’s structured illumination microscopy system and a Prime 95B camera with a 100X/1.47NA objective (top row) or 40x/1.3NA objective (bottom row). Images were reconstructed using SIMToolbox [2]. Conventional widefield fluorescence images (left) display out of focus light, raw SIM images (center) display the illumination pattern, and the 3D SIM reconstruction (maximum intensity projection, right) shows high quality, optically sectioned images.