Cameras for Light Sheet Microscopy

Author: Dan Croucher, Applications Team Manager, Teledyne Photometrics

Teledyne Photometrics Iris 15

The Teledyne Photometrics Iris 15 is a large format CMOS camera specifically designed for light sheet microscopy with a massive 25 mm diagonal field of view and small 4.25x4.25 µm pixels.

Light sheet microscopy is typically performed with low magnification objectives to maximize the field of view for imaging large samples. A larger field of view camera allows large samples to be imaged without needing to stitch multiple images together. Alternatively, for smaller samples, the large field of view can be split to perform multichannel imaging easily on the same sensor.

The small, 4.25x4.25 µm pixels achieve Nyquist sampling at 40x magnification, which gives improved resolution over typical sCMOS devices for all low magnification objectives.

Light sheet microscopy is designed to image dynamic events in live cells over long time scales. The high 73% peak quantum efficiency (QE) and low 1.5 e- read noise maximizes dim signal detection and allows for short exposure times to minimize the photodamaging effect of light on samples. The high QE is maintained far into the red and infra-red which is perfect for light sheet applications that image deep into cells and tissues.

Finally, images can be acquired at 32 frames per second at full frame allowing dynamic events to be visualized with high speed on a massive field of view.

Teledyne Photometrics Prime 95B

The Teledyne Photometrics Prime 95B is a high sensitivity CMOS camera designed for imaging at extremely low light with high speed and low noise.

Light sheet microscopy is considerably less damaging to live cells than other forms of fluorescence microscopy which enables samples to be imaged over very long time scales. The almost perfect, 95% QE sensor of the Prime 95B allows light intensity to be reduced even further while maintaining a high level of signal detection. This is ideal for reducing the effects of photobleaching as well as improving the condition of samples that are particularly sensitive to photodamage.

Compared to typical sCMOS devices, the exposure time on the Prime 95B could be reduced by up to four times and still give equivalent detection. It also maintains the large field of view (18.66 mm diagonal) and high speed (82 fps, full frame) expected of typical sCMOS devices. If a larger field of view is required, a version of the Prime 95B with a 25 mm diagonal is also available.