Prof. Kishan Dholakia
Dr. Stella Corsetti,
Optical Manipulation Group, School of Physics and Astronomy, University of St. Andrews
The Optical Manipulation Group at the University St. Andrews, led by Prof. Kishan Dholakia, looks at the science of light in terms of physics and biomedical research, fundamental and applied, in order to further the agenda for healthcare applications.
Prof. Dholakia wishes to take very high-quality images, in his terms: “A large field of view with high resolution, very quickly, very accurately, using the lowest possible photon dose.” For these reasons, the group uses light-sheet microscopy for the ability to take images with a lower light dose over a large field of view, with an aim to shed light on numerous biological processes.
With live samples, the need to avoid photodamage is necessary in order to avoid affecting biological functions, when samples need to be imaged for days. Prof. Dholakia outlined some other issues: “We had challenges in how do we create the field of view, and how do we do Nyquist sampling across this field of view succinctly and accurately.”
Previous CMOS solutions were not able to deliver the desired image quality, meaning that a new solution was needed in order to achieve the large field of view images while retaining enough sensitivity to use lower light levels and preserve live samples for longer, as well as the ability to image with high resolution at low magnifications.
What we found exciting was the very small pixel size [of the Iris 15], which means we can retain the resolution but still get the big picture.