The Effect of Camera Cooling on Signal to Noise Ratio
Cooling is a necessary feature on scientific CMOS cameras. Cooling directly reduces dark current, lowering the noise floor, as well as minimizing the occurrence of hot pixels. An uncooled scientific camera would not only struggle with low-light detection but, due to hot pixels, would also not perform as a true quantitative measurement device.
On an uncooled camera, hot pixels would otherwise need to be controlled by interpolation filters which can be problematic for some applications requiring quantitative pixel uniformity such as in super-resolution localization microscopy.