Alexandros Fragkopoulos, PhD
Oliver Bäumchen, PhD, Group Leader
Max Planck Institute for Dynamics and Self Organization
Physics of Soft and Living Matter, Bäumchen Lab, Göttingen
The Bäumchen lab is researching the physics underlying interfaces of soft and biological matter. In particular, they want to understand how those interfaces can alter the dynamics of soft and living matter. To achieve this, a multitude of techniques including micro- and nanofluidics, lab-on-a-chip technologies and force spectroscopy methods are employed. One of the lab’s model systems is the microalgae Chlamydomonas reinhardtii, for which the lab is studying flagella-mediated cell adhesion and motility, at interfaces which can be controlled by light.
To fully access the dynamics of the specimen of interest, a suspension of microalgae Chlamydomonas reinhardtii, Dr. Fragkopoulos, postdoc in the Bäumchen lab, looks into light-triggered behavior using high speed microscopy. The typical experiment includes a population of Chlamydomonas algae and documenting behavior over time. With their previous imaging solution, the lab did not have the camera sensitivity needed to record fast enough (>30 fps) at low-light conditions, nor did they have a suitable field of view (FOV) which meant having to produce extremely time-consuming imaging sessions.
Using the Iris 9 Scientific CMOS [sCMOS] camera, we are able to record videos of cells in low-light conditions with high enough resolution to identify individual cells, and with high enough speed to resolve their full range of motion with the camera’s large field of view.