Uri Manor, Biophotonics Core Director
Salk Institute for Biological Studies, Biophotonics Core Facility
The Salk Institute is home to a highly collaborative cadre of scientists who delve into a broad range of research areas, from aging, cancer and immunology to diabetes, brain science and plant biology. The group is supported by on-campus research centers and core facilities that are equipped with cutting-edge technology.
The Institute embodies Jonas Salk’s mission to dare to make dreams into reality by exploring the very foundations of life, seeking new realities in neuroscience, genetics, immunology and more. The team lives to discover, be it cancer or Alzheimer’s, aging or diabetes, they understand that every cure has a starting point. Salk is where cures begin.
Uri Manor, biophotonics core director works with the Salk researchers to provide collaborative support for a wide variety of research projects that require scientific imaging. Manor also works with the faculty steering committee to incorporate new and advanced imaging technologies into the repertoire of resources offered through the Biophotonics Core Facility.
Images depict the actin cytoskeleton as stained by AlexaFluor488-Phalloidin. EMCCD used was the Photometrics Evolve 512 and the Scientific CMOS was the Photometrics Prime 95B. Images were captured using Micro-Manager on a Zeiss Confocal microscope with a Yokogawa spinning disk scan head. 25ms and 50ms exposures were acquired with laser power set to the minimum of 5% with the EM Gain of the EMCCD set to 400.
The Facility provides technical and logistical access to Salk faculty, enabling the integration of imaging tools into a variety of biological research programs. To maintain its ability to advance science, the Facility must maintain the latest, cutting-edge commercial imaging and data analysis technologies available. This is especially important given that most projects involve sophisticated and complex research techniques.
The primary instrumentation that core researchers must have access to include technologies that support confocal microscopy (both fixed and live cell), TIRF microscopy, two-photon microscopy, electron microscopy and super-resolution microscopy as well as in-vivo imaging modalities.
The Prime 95B [Scientific CMOS camera] provides the speed, field of view and resolution of a CMOS camera, with the added sensitivity of an EMCCD camera for our more demanding experiments.