Outperforming EMCCD cameras, the Prime 95B Scientific CMOS (sCMOS) camera series includes several back illuminated sCMOS cameras that offer 95% QE with extreme sensitivity and high frame rates.
- 95% Quantum Efficiency
- 11 µm x 11 µm Pixel Area
- 3 Field of View Options
The Prime 95B back-illuminated sCMOS camera achieves a near-perfect 95% quantum efficiency.
By bringing the light in from the back of the sensor, photons land directly onto the light receiving surface, maximizing light collecting capability. Combining this with large, 11 µm pixels results in a camera technology as sensitive as EMCCD with greater stability, faster speeds and no gain aging.
Superior Background Quality
The Prime 95B features Pattern Noise Reduction Technology and Correlated Noise Reduction Technology to ensure that it delivers clean, pattern-free images with minimal pixel defects, delivering improved image quality in low light conditions.
Multiple Fields of View
The Prime 95B is available with an 18.8, 22 or 25 mm diagonal field of view. The larger format sensors are designed to increase throughput, maximize the amount of data captured and take full advantage of new, larger field of view microscopes.
The Prime 95B offers two data reduction technologies; PrimeLocate and Multi-ROI.
PrimeLocate can also be extended to offer Live Particle Tracking on the camera, giving early insight into the kinetics of moving molecules for single molecule tracking applications.
PrimeEnhance active denoising provides a real-time quantitative increase in signal to noise ratio by reducing the effects of photon shot noise at low light levels.
Sequenced Multiple Acquisition Real Time (SMART) Streaming enables the Prime 95B to capture a continuous sequence of images while cycling through 16 pre-programmed exposure time values, delivering the fastest multi-channel acquisition times.
The Prime 95B also supports up to four expose-out signals and has the capability for an effective global shutter.
|Pixel Size||11 µm x 11 µm|
|Supported Interfaces||PCIe and USB 3|
|Spectrum Capabilities||UV (200-440 nm), Visible (400-700 nm) |
Near Infrared (700-1000 nm)
|Read Noise (median)||1.6 e-|
18.7 mm diagonal
80fps @ 12-bit
22 mm diagonal
68fps @ 12-bit
25 mm diagonal
60fps @ 12-bit
Single Molecule TIRF Microscopy
“I find the performance of the Prime 95B [Scientific CMOS camera] to be comparable to state-of-the-art EMCCDs”
Microfluidic Chip Imaging of Live Yeast
“The bigger our field of view and the lower the exposure times are, the more cells we can image overall as less images are needed and the acquisition is faster [using the Prime 95B Scientific CMOS camera].”
Neuroscience and Calcium Imaging
“The Prime 95B [Scientific CMOS camera] allowed us to not only increase the frame rate we were using to acquire images, but we also achieved higher resolution. For us, that meant being able to look at subcellular structure in real time.”