Outperforming EMCCD cameras, the Prime 95B Scientific CMOS (sCMOS) camera series includes several back illuminated sCMOS cameras that offer 95% QE with extreme sensitivity and high frame rates.
- 95% Quantum Efficiency
- 11 µm x 11 µm Pixel Area
- 3 Field of View Options
The Prime 95B back-illuminated sCMOS camera achieves a near-perfect 95% quantum efficiency.
By bringing the light in from the back of the sensor, photons land directly onto the light receiving surface, maximizing light collecting capability. Combining this with large, 11 µm pixels results in a camera technology as sensitive as EMCCD with greater stability, faster speeds and no gain aging.
Superior Background Quality
The Prime 95B features Pattern Noise Reduction Technology and Correlated Noise Reduction Technology to ensure that it delivers clean, pattern-free images with minimal pixel defects, delivering improved image quality in low light conditions.
Multiple Fields of View
The Prime 95B is available with an 18.8, 22 or 25 mm diagonal field of view. The larger format sensors are designed to increase throughput, maximize the amount of data captured and take full advantage of new, larger field of view microscopes.
The Prime 95B offers two data reduction technologies; PrimeLocate and Multi-ROI.
PrimeLocate can also be extended to offer Live Particle Tracking on the camera, giving early insight into the kinetics of moving molecules for single molecule tracking applications.
PrimeEnhance active denoising provides a real-time quantitative increase in signal to noise ratio by reducing the effects of photon shot noise at low light levels.
Sequenced Multiple Acquisition Real Time (SMART) Streaming enables the Prime 95B to capture a continuous sequence of images while cycling through 16 pre-programmed exposure time values, delivering the fastest multi-channel acquisition times.
The Prime 95B also supports up to four expose-out signals and has the capability for an effective global shutter.
|Pixel Size||11 µm x 11 µm|
|Supported Interfaces||PCIe and USB 3|
|Spectrum Capabilities||UV (200-440 nm), Visible (400-700 nm) |
Near Infrared (700-1000 nm)
|Read Noise (median)||1.6 e-|
18.7 mm diagonal
82fps @ 12-bit
22 mm diagonal
70fps @ 12-bit
25 mm diagonal
60fps @ 12-bit
Low-Light, High-Speed FRET
“The Prime 95B lets us look at 5× the FOV at about twice the speed, but with the same sensitivity as our previous EMCCD system.”
Point Spread Function Engineering
“The high quantum yield for photon detection in the Prime 95B means that now we can be confident that we can detect almost every precious photon in our low signal measurements”
Structured Illumination Microscopy
“Imaging twice as fast with the Prime 95B [Scientific CMOS camera] is a great advantage. The reduction of pixel-to-pixel variance and the reduction of visible column structure in the camera greatly improves our results.”