Christopher Yip, Ph.D.
Director of the Institute of Biomaterials and Biomedical Engineering (IBBME), University of Toronto
Currently the Associate Vice President, International Partnerships at the University of Toronto, Dr. Christopher Yip is the former Director of the Institute of Biomaterials and Biomedical Engineering, and a faculty member in IBBME, Chemical Engineering, and Biochemistry, where he and his team create a variety of experiment-speciﬁc microscopy tools.
Dr. Yip told us, “Our lab core research focus is in single-molecule biophysics and the development of combinatorial imaging platforms for the study of molecular and cellular dynamics. We are particularly interested in the integration and application of different imaging modalities to understand protein-protein interactions on a wide range of temporal and spatial scales. A key platform for our lab is light sheet microscopy, and, we have been actively developing a diverse suite of systems, each tailored to a speciﬁc experiment. Some of our efforts include tracking receptor association, protein-membrane interactions, and dynamics. Our most recent work has focused on understanding the mechanisms of coordinated cell migration during heart development using Drosophila as the model system.”
In the development of light-sheet microscopy imaging for single molecule biophysics, many cameras require users to choose between a large ﬁeld of view, sensitivity, and high spatial resolution.
Dr. Yip explains, “We face a number of key imaging challenges in our research. The ﬁrst is low light levels as is often the case when studies are performed of low-expression proteins under endogenous promoter control. Additionally, the need for high spatial and temporal resolution is critical.”
Having the Iris 15’s high sensitivity, small pixels, and large field of view addresses a number of key experimental considerations for our study of development, including the ability to acquire overlapping images from different imaging angles, which is key for multi-view SPIM.