Anders Kyrsting, Post-doc
The Linke Group, University of Lund, Sweden
The Linke Group at the University of Lund, Sweden, creates artificial molecular motors and nanowires to better understand the role of biological motors in cellular processes such as cargo transportation, muscle contraction and cell division. The group tags the motors with quantum dots and images them using single molecule TIRF. They have further plans to expand their investigation with optical trapping and STORM super-resolution microscopy.
Anders Kyrsting, post-doc with the Linke Group, explained, “Molecular motors move very fast so to track them, we need a camera with a very high frame rate.” The group was previously using an EMCCD camera but the slower frame rate of its architecture meant that they had very limited temporal resolution.
Kyrsting continued, “Investigating single molecules means working with very low fluorescence signal. So, a camera with high sensitivity is equally as important as our need for a fast camera.” For this reason, the group couldn’t afford to sacrifice sensitivity for speed.
I don’t think I’d use an EMCCD again, I don’t know why I’d use it with the performance we get out of the Prime 95B [Scientific CMOS camera].