Prof. Dirk-Peter Herten
Professor Herten leads the Single-Molecule Spectroscopy group at Heidelberg University aiming at quantitative analytical approaches in biology and chemistry based on single-molecule data acquired with advanced fluorescence microscopy.
The groups research interests range from the development of fluorescent probes for live-cell microscopy to the investigation of chemical reactions on a single-molecule level. The group successfully studies relatively simple reactions like complexation of Cu(II), Cu(I)/Cu(II) redox reactions and epoxidation of aliphatic double bonds.
In the area of single-molecule chemistry, the group have now turned to more complex catalytic reactions to try to identify individual reaction steps and alternative reaction pathways.
Here, the limited observation time due to photo-bleaching and low photon yield poses a major obstacle in the investigation of chemical transitions.
The precise identification of different molecular states occurring randomly during a chemical transformation requires excellent signal to noise ratio at high time-resolution in the millisecond range.
The Prime 95B, with the combination of large pixels and back-illumination, achieves not only a very high quantum yield but also a low readout noise at very high frame rates.