Live CellFluorescence Microscopy

Live Cell Imaging

Live cell imaging gives us the ability to observe dynamic processes in cells, tissues, or whole organisms as they happen. Compared to fixed cells, live cells provide us with information about the changes that occur in the cell during processes necessary for life. This includes everything from cell division to cell migration, movements and transformations of organelles and calcium imaging. 

Our range of cameras for live cell imaging ensures that you’ll find the combination of specifications that match your requirements.

Prime BSI photo

Prime BSI

High sensitivity, 95% quantum efficient, sCMOS camera with 6.5 µm pixels and 1.0 e read noise.

The back-illuminated Prime BSI is particularly suitable for low light fluorescence imaging. The high quantum efficiency combined with the balanced 6.5 µm pixel size allows for Nyquist sampling with the most popular objective magnifications used for live cell imaging.

With live imaging possible at over 60 fps full frame, users can rapidly find and focus on their samples and capture images using the shortest exposure times to minimize sample photodamage.

Live Cell Imaging Samples

Prime 95B

Highest sensitivity, 95% quantum efficient sCMOS camera with 11 µm pixels and EMCCD level detection.

Maximize sensitivity with the back-illuminated Prime 95B to observe the most demanding low light cell samples. Pairing the almost-perfect 95% quantum efficiency with a large 11 µm pixel allows exposure times to be lowered significantly to reduce photobleaching and photodamage to the lowest levels possible on an sCMOS camera.

Live Cell Samples

Iris 9

High resolution, 4.25 µm pixel sCMOS camera with 9 million pixels.

Maximize resolution with the Iris 9, perfect for when the highest level of image detail is required. Live cell imaging is possible at over 30 fps full frame with a high, broadband quantum efficiency to image dyes with the most challenging emission wavelengths.

Live Cell Samples

Customer Stories

Microfluidic Chip Imaging of Live Yeast

Dr. Marc Fouet University of California, Berkeley

“The bigger our field of view and the lower the exposure times are, the more cells we can image overall as less images are needed and the acquisition is faster [using the Prime 95B Scientific CMOS camera].”

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Live Vesicle Trafficking

Drosophila epithelial cells. The arrows indicate vesicles containing a cargo protein, cell adhesion molecule Fasciclin III (FAS3) tagged with Halo and labeled with SiR dye.
Dr. George Sirinakis University of Cambridge

“The high sensitivity and low read noise of the Prime BSI is transformative in these experiments as it enables us to use low levels of excitation power and significantly reduce photo-damage to the tissue.”

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Widefield Fluorescence Microscopy

Mouse retina displaying the layer stratification of cell types and subregions
Dr. Steven Pittler University of Alabama at Birmingham

“At 9MP resolution, the Iris 9 image quality is spectacular and allows for the capture of very fine detail”

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