Neuroscience and ElectrophysiologyFluorescence Microscopy
Neuroscience and Electrophysiology
Electrophysiology is a method that allows us to study the function of electrically active cells, such as neurons. Research on the physiology of neurons and the nervous system is often accomplished at the cellular level using microscopy techniques or further techniques such as patch clamping.
Advanced imaging methods in neuroscience permit us to image deep into brain tissue with high spatial and temporal resolution. It is even possible, using advanced optogenetic methods, to optically interrogate cells to discover more about their function.
Cameras for neuroscience applications can have very different specification requirements based on what is being investigated. Cellular activity as a response to electrical stimulation or changes to membrane potentials, for example, can happen extremely quickly, so high frame rates can be of high importance. Many dyes used in electrophysiology are difficult to visualize because of a low quantum efficiency at the fluorophores emission wavelength. Therefore, a camera with a high quantum efficiency over the entire visible wavelength range may also be desired.
Finally, electrophysiology applications often require minimal electronic noise and vibrations to acquire high-quality data so the electrophysiologist requires tight control of camera noise.
The Retiga ELECTRO is specifically designed for electrophysiology.
The high broadband quantum efficiency delivers the sensitivity required for high contrast imaging of cells and tissues, making the manipulation of micropipettes in their contact with cell membranes and tissues significantly easier to perform.
Fanless temperature regulation using a thermoelectric cooling system cools the sensor to 0oC without adding additional vibration to the system. This means that electrophysiology experiments no longer need to compensate for this additional noise.
Incorporated grounding pins allow the camera to be grounded within the rig to reduce the buildup of static electricity in electrical systems.
Finally, in-built Intelligent Quantification and Defective Pixel Correction technologies improve signal to noise for long exposures.
High sensitivity, 95% quantum efficient, sCMOS camera with 6.5 µm pixels and 1.0 e– read noise.
The Prime BSI delivers the perfect balance between high-resolution imaging, sensitivity and speed. Nyquist spatial sampling is achieved at 60x magnification with no optical correction and the 45,000 e- linear full well capacity is 50% higher than other sCMOS cameras for the greatest dynamic range.
Read noise is minimized to just 1.0 e-, ideal for visualizing the smallest change in signal intensity, and with sCMOS speed the fastest events can be captured.
When the ELECTRO isn’t enough, the Prime BSI provides the necessary boost in sensitivity and speed.
Highest sensitivity, 95% quantum efficient sCMOS camera with 11 µm pixels
The Prime 95B is our highest sensitivity sCMOS device for the ultimate in high signal to noise and high contrast imaging with ~2.8x higher sensitivity than the Prime BSI and ~3.3x higher sensitivity than other sCMOS cameras.
Compared to typical sCMOS devices, the exposure time on the Prime 95B could be reduced by up to four times and still give equivalent detection, increasing speed and minimizing the effects of photobleaching and photodamage.
“The Prime 95B [Scientific CMOS camera] allowed us to not only increase the frame rate we were using to acquire images, but we also achieved higher resolution. For us, that meant being able to look at subcellular structure in real time.”
“The camera has allowed us to acquire images that would otherwise be impossible to obtain, providing a basis for future analysis of how the properties of capillaries, and of red and white blood cells, determine brain blood flow.”