PRIME BSI95% Quantum Efficiency

The Prime BSI Scientific CMOS (sCMOS) camera delivers the perfect balance between high resolution imaging and sensitivity with an optimized pixel design and near perfect 95% Quantum Efficiency.

  • 95% Quantum Efficiency
  • 6.5 µm x 6.5 µm Pixel Area
  • 1 e- Read Noise

Key Features

QE Curve

Sensitivity

The Prime BSI back-illuminated sCMOS camera achieves a near-perfect 95% quantum efficiency. By bringing the light in from the back of the sensor, photons land directly onto the light receiving surface, maximizing light collecting capability. The Prime BSI combines 95% quantum efficiency with a low 1.0 e- read noise to deliver the most sensitive camera based on sCMOS technology at over 40 frames per second.

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Testes application image

Resolution and Pixel Size

The Prime BSI features 6.5 μm pixels, the accepted standard for most live cell applications using 40x and 60x magnification. This pixel size provides highly detailed images across the imaging plane and is most suitable for the broadest range of microscope objectives.

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Superior Background Quality

The Prime BSI features Pattern Noise Reduction Technology and Correlated Noise Reduction Technology to ensure that it delivers clean, pattern free images with minimal pixel defects, delivering improved image quality in low light conditions.

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Programmable Scan Mode image

Advanced Triggering

Programmable Scan Mode provides increased control over the rolling shutter exposure and read-out functionality of CMOS sensors for applications that require control over the line time.

Sequenced Multiple Acquisition Real Time Streaming (SMART) Streaming enables the Prime BSI to capture a continuous sequence of images while cycling through 16 pre-programmed exposure time values, delivering the fastest multi-channel acquisition times.

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Advanced Features

The Prime BSI offers PrimeEnhance active denoising which provides a real-time quantitative increase in the signal to noise ratio to maximize sensitivity at ultra-low light levels.

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Family Specifications

Sensor Technology sCMOS
Quantum Efficiency 95%
Pixel Size 6.5 µm x 6.5 µm
Supported Interfaces PCIe and USB 3
Spectrum Capabilities UV (200-400 nm), Visible (400-700 nm)
Near Infrared (700-1000 nm)
Read Noise (median) 1.0 e-
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Camera Models

Model
Megapixels
Sensor Area
Frame Rate
Prime BSI
Megapixels
4.2
Sensor Area
13.3 mm x 13.3 mm
18.8 mm diagonal
Frame Rate
43 fps @16-bit /
63 fps @ 11-bit

Downloads

Datasheet
Type
Document
Manuals
Document
Handbook
Document

Customer Stories

Optogenetics and Fluorescent Protein Imaging

Dr. Scott Cruikshank Brown University

“Using the Prime BSI to image weakly illuminated and sparsely labeled fluorescent neurons, we are able to avoid uncontrolled activation of channelrhodopsin while imaging the signal in axon terminals using short exposure times”

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Imaging with Quantum Light Source

Dr. Markus Gräfe Fraunhofer Institute

“The pixel size of the Prime BSI is great, it has much smaller pixels compared to an EMCCD so it can give us much better resolution which has been a huge benefit to us. The camera itself is also much smaller which is ideal for making the system more compact”

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Super Resolution Imaging

Comparison of live cell imaging of FtsZ ring organization during bacterial cell division using a Prime BSI and an EMCCD camera.
Dr. Seamus Holden Newcastle University

“The thing that really impressed me is how uniform the sensor is – far fewer hot pixels, noisy pixels and stripes than the last generation of sCMOS cameras. I hardly see a use for EMCCDs anymore.”

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