The Prime BSI Scientific CMOS (sCMOS) camera delivers the perfect balance between high resolution imaging and sensitivity with an optimized pixel design and near perfect 95% Quantum Efficiency.
- 95% Quantum Efficiency
- 6.5 µm x 6.5 µm Pixel Area
- 1 e- Read Noise
The Prime BSI back-illuminated sCMOS camera achieves a near-perfect 95% quantum efficiency. By bringing the light in from the back of the sensor, photons land directly onto the light receiving surface, maximizing light collecting capability. The Prime BSI combines 95% quantum efficiency with a low 1.0 e- read noise to deliver the most sensitive camera based on sCMOS technology at over 40 frames per second.
Resolution and Pixel Size
The Prime BSI features 6.5 μm pixels, the accepted standard for most live cell applications using 40x and 60x magnification. This pixel size provides highly detailed images across the imaging plane and is most suitable for the broadest range of microscope objectives.
Superior Background Quality
The Prime BSI features Pattern Noise Reduction Technology and Correlated Noise Reduction Technology to ensure that it delivers clean, pattern free images with minimal pixel defects, delivering improved image quality in low light conditions.
Programmable Scan Mode provides increased control over the rolling shutter exposure and read-out functionality of CMOS sensors for applications that require control over the line time.
Sequenced Multiple Acquisition Real Time Streaming (SMART) Streaming enables the Prime BSI to capture a continuous sequence of images while cycling through 16 pre-programmed exposure time values, delivering the fastest multi-channel acquisition times.
The Prime BSI offers PrimeEnhance active denoising which provides a real-time quantitative increase in the signal to noise ratio to maximize sensitivity at ultra-low light levels.
|Pixel Size||6.5 µm x 6.5 µm|
|Supported Interfaces||PCIe and USB 3|
|Spectrum Capabilities||UV (200-400 nm), Visible (400-700 nm) |
Near Infrared (700-1000 nm)
|Read Noise (median)||1.0 e-|
18.8 mm diagonal
63 fps @ 11-bit
Optogenetics and Fluorescent Protein Imaging
“Using the Prime BSI to image weakly illuminated and sparsely labeled fluorescent neurons, we are able to avoid uncontrolled activation of channelrhodopsin while imaging the signal in axon terminals using short exposure times”
Imaging with Quantum Light Source
“The pixel size of the Prime BSI is great, it has much smaller pixels compared to an EMCCD so it can give us much better resolution which has been a huge benefit to us. The camera itself is also much smaller which is ideal for making the system more compact”
Super Resolution Imaging
“The thing that really impressed me is how uniform the sensor is – far fewer hot pixels, noisy pixels and stripes than the last generation of sCMOS cameras. I hardly see a use for EMCCDs anymore.”