Prime 95B™ Scientific CMOS (sCMOS) Camera Series

Prime 95B Camera
  • 95% Quantum Efficiency
  • 11µm x 11µm Pixel Area
  • Real-time Computational Capabilities
  • 3 Field of View Options

Outperforming EMCCD cameras, the Prime 95B Scientific CMOS (sCMOS) Camera Series includes several back illuminated sCMOS cameras that offer 95% QE with extreme sensitivity and high frame rates. A large field of view (FOV) maximizes imaging throughput on larger format microscopes. Proprietary PrimeEnhance and PrimeLocate advanced imaging features benefit super-resolution microscopy, single molecule fluorescence and confocal microscopy.

Prime 95B field of view sample
Fits 19 mm C-mount microscope ports
Prime 95B 22mm field of view sample
Fits 22 mm C-mount microscope ports
Prime 95B 25mm field of view sample
Fits 25 mm F-mount microscope ports
PrimeEnhance - before PrimeEnhance - after

Prime 95B is available with an embedded signal processing engine that quantitatively restores images that are hidden in photon noise. Learn more these advanced, real‐time processing features:

95% Quantum Efficiency comparison graph
Features   Prime 95B EMCCD
Fast Acquisition >82fps @ 1200 x 1200  
High Spatial Resolution Optimized for 100X Imaging  
Large Field of View Maximizes Imaging Throughput  
16-bit Dynamic Range Simultaneously Image Bright & Dim Samples  
Excess Noise Factor 1.4X Higher Shot Noise  
EM Gain Degrades over time  

VIEW WEBINAR: A new generation of sensors and signal processing

Learn more about the Prime family of Scientific CMOS Cameras

  • 95% Quantum Efficiency
  • 11µm x 11µm Pixel Area
  • 1200 x 1200 array - 1.44 Megapixel
  • 1.6e- Read Noise
  • 80,000e- Pixel Full Well
  • 61,500:1 Dynamic Range
  • 41fps @ 16-bit
  • 82fps @ 12-bit

The Prime 95B lets us look at 5× the FOV at about twice the speed, but with the same sensitivity as our previous EMCCD system.

Professor Andrew Plested, Leibniz Research Institute for Molecular Pharmacology, Humboldt University Berlin

 

We’ve been using the Prime 95B as one of our main cameras for both single molecule sensing and nano-bead velocity measurements. The Prime 95B Scientific CMOS (sCMOS) is excellent for low-noise, high-sensitivity, and fast measurements.

Dr. Kevin Freedman, University of California Riverside

 

Compared to the EMCCD, the Prime 95B Scientific CMOS (sCMOS) gives us a field of view large enough for our most demanding experiments as well as faster frame rates.

Dr Thomas Etheridge, University of Sussex

 

Better temporal and spatial resolution means better quality data. We’ll definitely be using the Prime 95B Scientific CMOS (sCMOS) camera for our upcoming experiments.

Dr. Christoph Baumann, University of York

 

Altogether, there is no better camera for cutting-edge single molecule microscopy than the Prime 95B Scientific CMOS (sCMOS)

Prof. Mark Leake, University of York

 

The Prime 95B Scientific CMOS (sCMOS) camera provides the required sensitivity that is needed to image our yeast cells with as little excitation light as possible.

Christof Osman, Ludwig-Maximilian University

 

The quantum efficiency of the camera is a really important factor for us. If we can use less excitation power, we can increase the length of our imaging and minimize photodamage to the animal. The sensitivity of the Prime 95B Scientific CMOS (sCMOS) camera is truly transformative for our type of work.

Shiaulou Yuan, Yale University

 

The Prime 95B Scientific CMOS (sCMOS) is an outstanding camera, particularly due to its low-noise, high-sensitivity and large field-of-view.

Dr. Ricardo Henriques, University College London

 

The Prime 95B Scientific CMOS (sCMOS) camera is, right now, the best solution we have found for TILT imaging. Coupling the Prime 95B to the TILT generates an extremely powerful imaging system!

Dr. Paul Maddox, University of North Carolina

 

The high QE of the Prime 95B Scientific CMOS (sCMOS) camera will allow us to improve our investigation of protein dynamics and extend single molecule tracking to more challenging samples.

Dr. Martin Lenz, University of Cambridge

 

The high speed and large field of view of the Prime 95B Scientific CMOS (sCMOS) camera are a massive advantage for our work.

Madhavi Krishnan, Krishnan Group, University of Zürich

 

The bigger our field of view and the lower the exposure times are, the more cells we can image overall as less images are needed and the acquisition is faster [using the Prime 95B Scientific CMOS (sCMOS) camera].

Marc Fouet, University of California, Berkeley

 

The Prime 95B camera is specified to provide >95% quantum efficiency, giving us the advantages of Scientific CMOS (sCMOS) with fantastic sensitivity.

Paul French, Imperial College London, Physics Department

 

The Prime 95B Scientific CMOS (sCMOS) camera allowed us to acquire high contrast fluorescence images using low excitation intensities, and subsequently allowed us to image over longer periods of time and at higher frequencies than previously possible. This allowed for intrusion-free study of many sensitive developmental processes.

Simon Berger, deMello Group, ETH Zürich (Switzerland)

We can now more precisely locate each fluorescent dye that is targeting a protein within a complex [with the Prime 95B Scientific CMOS (sCMOS) camera]. This has the effect of improving the resolution of our structural models, allowing us to see details inside these complexes that we could not before.

Dr. Kyle M. Douglass, Suliana Manley Lab

 

I think the Prime 95B Scientific CMOS (sCMOS) camera is a very innovative product. Partly because of the back thinning but also because the pixel size is more appropriate than conventional Scientific CMOS cameras.

Simon Watkins, University of Pittsburgh, Center for Biologic Imaging

 

The Prime 95B Scientific CMOS (sCMOS) camera provides the speed, field of view and resolution of a CMOS camera, with the added sensitivity of an EMCCD camera for our more demanding experiments.

Uri Manor, SALK Institute for Biological Studies, Biophotonics Core Facility

 

[Prime 95B Scientific CMOS (sCMOS) camera] allows us to conduct our STORM experiments with higher frame rates… 95 percent quantum efficiency allows for super-resolution imaging that’s not achievable with conventional sCMOS cameras.

Ke Xu, University of California Berkeley, College of Chemistry

 

For single-molecule images like in DNA-PAINT, the Prime 95B Scientific CMOS (sCMOS) camera combined with PrimeEnhance allows us to conduct super-resolution imaging with higher spatial resolution than that of EMCCD technology.

Sang-Hee Shim, Institute for Basic Science, Korea University

 

Imaging twice as fast with the Prime 95B Scientific CMOS (sCMOS) camera is a great advantage. The reduction of pixel-to-pixel variance and the reduction of visible column structure in the camera greatly improves our results.

Guy Hagen, University of Colorado, Colorado Springs

 

If you have a shorter exposure time, you can track faster kinetics. More sensitivity and shorter exposure times with the Prime 95B Scientific CMOS (sCMOS) camera allow you to image faster and track kinetics better.

Yandong Yin, New York University, School of Medicine

 

I don't think I’d use an EMCCD again, I don’t know why I'd use it with the performance we get out of the Prime 95B Scientific CMOS (sCMOS) camera.

Anders Kyrsting, The Linke Group, University of Lund, Sweden

 

I find the performance of the Prime 95B Scientific CMOS (sCMOS) camera to be comparable to state-of-the-art EMCCDs

Aleks Ponjavic, Klenerman Group, University of Cambridge

 

The Prime 95B Scientific CMOS (sCMOS) camera allowed us to not only increase the frame rate we were using to acquire images, but we also achieved higher resolution. For us, that meant being able to look at subcellular structure in real time.

Geoffrey G. Murphy, University of Michigan

 

The Prime 95B Scientific CMOS (sCMOS) camera is the perfect camera for Spinning Disk - the image quality is a big improvement over our EMCCDs, and the field of view makes samples much easier to find.

Peter March, University of Manchester, Bioimaging Facility

 

Photometrics cameras are reliable with great performance. We can now visualize things what we could not see before [with the Prime 95B Scientific CMOS (sCMOS) camera].

Dr. Felix Evers, Centre for Organismal Studies, University of Heidelberg

 

…if you’re looking for a high performance camera, the Prime 95B Scientific CMOS (sCMOS) camera is definitely worth checking out. It lives up to the hype!

Kurt Thorn, UCSF


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