Prime 95B Scientific CMOS Camera

Prime 95B Scientific CMOS Camera

95% QE Backside Illuminated Scientific CMOS, when discovery depends on every photon

Developed with award-winning technology, the Prime 95B Scientific CMOS camera allows you to overcome many difficult imaging challenges by converting nearly every available photon to useful signal through an incredible 95% Quantum Efficiency – by using the first available scientific CMOS sensor with backside illumination. And now, the Prime 95B is available with advanced real-time processing features:

  • PrimeEnhance™ quantitatively increases the Signal to Noise Ratio by 3X-5X, increasing the clarity and quality of images.
  • PrimeLocate™ dynamically evaluates acquired images and reduces the surplus of data generated during high speed super-resolution imaging.

PrimeEnhance - before PrimeEnhance - after

Prime 95B is available with an embedded signal processing engine that quantitatively restores images that are hidden in photon noise. Learn more these advanced, real‐time processing features:

95% Quantum Efficiency comparison graph
Features   Prime 95B EMCCD
Fast Acquisition >82fps @ 1200 x 1200  
High Spatial Resolution Optimized for 100X Imaging  
Large Field of View Maximizes Imaging Throughput  
16-bit Dynamic Range Simultaneously Image Bright & Dim Samples  
Excess Noise Factor 1.4X Higher Shot Noise  
EM Gain Degrades over time  

VIEW WEBINAR: A new generation of sensors and signal processing

Learn more about the Prime family of Scientific CMOS Cameras

  • 95% Quantum Efficiency
  • 11µm x 11µm Pixel Area
  • 1200 x 1200 array - 1.44 Megapixel
  • 1.6e- Read Noise
  • 80,000e- Pixel Full Well
  • 61,500:1 Dynamic Range
  • 41fps @ 16-bit
  • 82fps @ 12-bit

The high speed and large field of view of the Prime 95B are a massive advantage for our work.

Madhavi Krishman, Krishnan Group, University of Zürich


The bigger our field of view and the lower the exposure times are, the more cells we can image overall as less images are needed and the acquisition is faster.

Marc Fouet, University of California, Berkeley


The Prime 95B is the perfect camera for Spinning Disk - the image quality is a big improvement over our EMCCDs, and the field of view makes samples much easier to find.

Peter March, University of Manchester, Bioimaging Facility


I find the performance of the Prime 95B to be comparable to state-of-the-art EMCCDs

Aleks Ponjavic, Klenerman Group, University of Cambridge


If you have a shorter exposure time, you can track faster kinetics. More sensitivity and shorter exposure times with the Prime 95B allow you to image faster and track kinetics better.

Yandong Yin, New York University, School of Medicine


The Prime 95B camera is specified to provide >95% quantum efficiency, giving us the advantages of Scientific CMOS with fantastic sensitivity.

Paul French, Imperial College London, Physics Department


Photometrics cameras are reliable with great performance. We can now visualize things that we could not see before.

Dr. Felix Evers, Centre for Organismal Studies, University of Heidelberg


I don't think I'd use an EMCCD again, I don't know why I'd use it with the performance we get out of the Prime 95B.

Anders Kyrsting, The Linke Group, University of Lund, Sweden


…if you’re looking for a high performance camera, the Prime95B is definitely worth checking out. It lives up to the hype!

Kurt Thorn, UCSF

I think the Prime 95B is a very innovative product. Partly because of the back thinning but also because the pixel size is more appropriate than conventional Scientific CMOS cameras.

Simon Watkins, University of Pittsburgh, Center for Biologic Imaging


The Prime 95B provides the speed, field of view and resolution of a CMOS camera, with the added sensitivity of an EMCCD camera for our more demanding experiments.

Uri Manor, SALK Institute for Biological Studies, Biophotonics Core Facility


The Prime 95B allowed us to acquire high contrast fluorescence images using low excitation intensities, and subsequently allowed us to image over longer periods of time and at higher frequencies than previously possible.

Simon Berger, deMello Group, ETH Zürich (Switzerland)


For single-molecule imaging, the EMCCD might still be the king…But for all other imaging and spinning disk confocal, I'd rather have the Prime 95B. No more deciding the optimal EM gain settings and the large dynamic range of the CMOS make it a real winner!

Sam Lord, Everyday Scientist


[Prime 95B] allows us to conduct our STORM experiments with higher frame rates… 95 percent quantum efficiency allows for super-resolution imaging that's not achievable with conventional Scientific CMOS cameras.

Ke Xu, University of California Berkeley, College of Chemistry


For single-molecule images like in DNA-PAINT, the Prime 95B combined with PrimeEnhance allows us to conduct super-resolution imaging with higher spatial resolution than that of EMCCD technology.

Sang-Hee Shim, Institute for Basic Science, Korea University


Imaging twice as fast with the Prime 95B is a great advantage. The reduction of pixel-to-pixel variance and the reduction of visible column structure in the camera greatly improves our results.

Guy Hagen, University of Colorado, Colorado Springs

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