OpenSPIM

sCMOS Optimised For Light Sheet Microscopy

Kinetix photo

Kinetix

  • Large FOV, no more stitching and tiling
  • Very high speed for live samples
  • Flexible and powerful light-sheet solution

The Kinetix delivers everything you need for light-sheet in one camera, with the highest speeds and largest field of view for high-throughput.

Iris 15

  • Large rectangular FOV for large samples
  • Small pixel ideal for low magnification
  • Sub-cellular resolution

The Iris 15 is ideal for imaging large fields of view at high resolution, easy to set up, and simple to image with.

Prime BSI Express

  • Ideal for higher magnification imaging
  • High sensitivity with high speed
  • Compact and easy to integrate

The Prime BSI Express is a sensitive, compact camera that images at high resolution and high speed.

OpenSPIM Customer Stories

Dr. Rui Ma, Max Planck Institute

Zebrafish Light-Sheet with the Kinetix sCMOS

“This light-sheet system was built with the Kinetix in mind, we wanted to use the larger field of view, higher QE and faster framerates, compared with other cameras.”

Dr. Franziska Decker, ETH Zürich

Organoid Light-Sheet with the Iris 15 sCMOS

“Every sample I have imaged fits into one field of view, and [the Iris 15] works great, it was easy to setup and image with.”

Dr. Greg Perry, University of London

3D organism imaging with the Iris 9 sCMOS

“The camera is ideal for our OpenSPIM system due to the sensitivity and the large field of view”

Want To Learn More?

Light-Sheet Customer Stories

Light Sheet

Prof. Kishan Dholakia University of St. Andrews

“What we found exciting was the very small pixel size [of the Iris 15], which means we can retain the resolution but still get the big picture.”

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mesoSPIM Light Sheet

Dr. Fabian Voigt University of Zurich

“We tested the Iris 15 and found its large detector size and rectangular sensor ideal for our applications.”

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eSPIM Light Sheet

Prof. Bo Huang University of California San Francisco

“The combination of sensitivity, speed, field of view size and robust camera engineering of the Prime BSI has been very beneficial to our large-scale live cell imaging experiments.”

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RETIGA E7Long Exposure CMOS

The Retiga E7 brings long exposure imaging into the CMOS era, featuring major breakthroughs in thermal noise control that allows for exposures of over 20 minutes! This compact CMOS has low dark noise and ultra-low dark current across a 7 megapixel sensor, resulting in high resolution detection of even the weakest signals. With data over a single USB3.2 Gen 2 cable, the Retiga E7 is simple, powerful and easy to integrate, representing the next generation of long exposure camera, following on from the highly successful CCD-based Retiga R-series.

  • Capable of exposure times over 20 minutes
  • Ultra low dark current
  • 7 Megapixels
  • Low noise

Key Features

Long Exposure Imaging

Thanks to major breakthroughs in thermal noise control, the Retiga E7 CMOS has an unprecedented ultra-low dark current of 0.02 electrons per second, allowing for exposure times of over 20 minutes.

Stretching exposure times from seconds to minutes in this manner results in comfortable detection of even the weakest signals.

High Resolution

The Retiga E7 features a 7 megapixel sensor made up of 4.5 μm pixels in a 3200 x 2200 array, well suited to high resolution imaging across a 17.6 mm diagonal. This combination of large field of view and small pixel size will capture fine details across even large samples.

High Speed Mode

As well as the ‘Long Exposure’ mode, capable of exposures over 20 minutes thanks to the ultra-low dark current, the Retiga E7 also features a ‘Fast Capture’ mode that operates at over 50 frames a second across the full 7 megapixel sensor.

OEM Integration

Interfacing is simple with the Retiga E7 by design. With its compact format and single cable interface, hardware integration is straightforward. The addition of onboard triggering, Windows/Linux support, and our dedicated OEM team make integrating and customising the Retiga E7 a painless process.

Family Specifications

Sensor Technology CMOS
Dark Current Noise 0.02 e-/p/s
Quantum Efficiency 73%
Pixel Size 4.5 µm x 4.5 µm
Supported Interfaces USB 3.2 Gen 2
Read Noise (median) 2.2 e-

Camera Models

Model
Megapixels
Sensor Area
Frame Rate
Retiga E7
Megapixels
7
Sensor Area
14.4mm x 9.9mm,
17.6mm diagonal
Frame Rate
3fps @ ‘Long Exposure’
50fps @ ‘Fast Capture’

Downloads

Datasheet
Type

KINETIX22High Speed, Back-Illuminated

 

The back-illuminated Kinetix22 Scientific CMOS (sCMOS) camera delivers high speed imaging with an optimized field of view for most modern microscopes, the most balanced pixel size and near perfect 95% quantum efficiency.

  • 22 mm Field Of View
  • 5.76 Megapixel
  • 2400 x 2400 6.5 μm Pixels
  • 498 Frames Per Second
  • 95% Quantum Efficiency
  • 0.7 e- Read Noise

 

Key Features

Ultimate Flexibility

The Kinetix22 features four modes, allowing users to prioritize different camera specs, optimise imaging, and avoid compromise.

The 8 bit Speed mode offers 498 fps across the full frame, 12 bit Sensitivity mode offers low 1.2 e- read noise while operating at ~90 fps, 16 bit Dynamic range has large full well capacity for varied signal levels in the sample, and 16 bit Sub-Electron mode offers EMCCD like sensitivity with 0.7 e- read noise.

Extreme Speed

The Kinetix22 delivers a tremendous 498 frames per second (fps), full frame across the entire 22 mm diagonal field of view. This speed significantly outperforms typical sCMOS devices while featuring a higher megapixel sensor.

The optimized line time results in a data rate of almost 3000 megapixels/second, around 7x greater than typical sCMOS devices.

Maximizing Signal Collection

The Kinetix22 back-illuminated camera achieves a near-perfect 95% quantum efficiency.

By bringing the light in from the back of the sensor, photons land directly onto the light receiving surface, maximizing light collecting capability. This allows for the weakest signals to be displayed as high quality image, even if your sample typically only emits a handful of photons.

Minimizing Noise

The Kinetix22 includes Sub-Electron mode, which has read noise levels around 0.7 e-.  In this mode, multiple measurements of the pixel value are taken, providing reduced noise from the camera. In addition, Sub-Electron mode uses a high sensitivity gain, so low signal levels provide great image contrast. With the low camera read noise in other modes, the Kinetix family represents the most versatile cameras on the market.

Testes samle

High Resolution

The Kinetix22 features 6.5 µm x 6.5 µm pixels, the accepted standard for most live cell applications using 40x and 60x magnification.

This pixel size provides highly detailed images across the imaging plane and is most suitable for the broadest range of microscope objectives.

Superior Background Quality

The Kinetix22 features Pattern Noise Reduction Technology and Correlated Noise Reduction Technology to ensure that it delivers clean, pattern-free images with minimal pixel defects, delivering improved image quality in low light conditions.

Family Specifications

Sensor Technology sCMOS
Quantum Efficiency 95%
Pixel Size 6.5 µm x 6.5 µm
Supported Interfaces PCIe and USB 3.2
Spectrum Capabilities UV (200-400 nm), Visible (400-700 nm),
Near Infrared (700-1000 nm)
Read Noise (median) 0.7 e-
Download full specifications

Camera Models

Model
Megapixels
Sensor Area
Frame Rate
Kinetix22
Megapixels
5.7
Sensor Area
15.56 mm x 15.56 mm
22 mm diagonal
Frame Rate
498 fps @ 8-bit
88 fps @ 12-bit
83 fps @ 16-bit
5 fps @ 16-bit
Model
Megapixels
Sensor Area
Frame Rate
Kinetix
Megapixels
10.2
Sensor Area
20.8 mm x 20.8 mm
29.4 mm diagonal
Frame Rate
498 fps @ 8-bit
88 fps @ 12-bit
83 fps @ 16-bit
5 fps @ 16-bit

SPECTRA X®
Light EngineVersatile and Powerful Fluorescence Illumination

SPECTRA X®

Versatile and Powerful Fluorescence Illumination

Unlock advanced applications in fluorescence with the SPECTRA X®. This 7 channel light engine provides ultimate control over illumination with internal easy-swap excitation filters, individual intensity control over every channel via software, and high-speed TTL interface for synchronizing with other components

  • 7 Channel emission
  • Advanced Control
  • High Speed

Key Features

7 Discrete Spectral Bands

Whatever fluorophore you intend to use, the SPECTRA X® can bring it to life with its broad spectral range.

Advanced Control

Individually control intensities of every illumination channel to fine-tune your experiment. And optional manual control pod makes working with the microscope eyepieces simpler.

High Illumination Power

High maximum illumination power combined with the high-speed switching, this light engine unlocks more demanding fluorescence applications

Individual Filtering

Have ultimate control over your illumination wavelengths with internal paddle-mounted filters with tool-free access.

High Speed

When synchronizing with a camera or other devices, fast turn-on and high speed switching capability is a must. The SPECTRA X® boasts 10µs on time and 5-10 kHz switching rate, enabling high speed applications.

Resources

Datasheet
Type
Document
Manual
Document
Technical Note
Document

SOLA SE®
Light EngineUltra-bright Fluorescence Illumination

SPECTRA X®

Versatile and Powerful Fluorescence Illumination

Unlock advanced applications in fluorescence with the SPECTRA X®. This 7 channel light engine provides ultimate control over illumination with internal easy-swap excitation filters, individual intensity control over every channel via software, and high-speed TTL interface for synchronizing with other components

  • 7 Channel emission
  • Advanced Control
  • High Speed

Key Features

7 Discrete Spectral Bands

Whatever fluorophore you intend to use, the SPECTRA X® can bring it to life with its broad spectral range.

Advanced Control

Individually control intensities of every illumination channel to fine-tune your experiment. And optional manual control pod makes working with the microscope eyepieces simpler.

High Illumination Power

High maximum illumination power combined with the high-speed switching, this light engine unlocks more demanding fluorescence applications

Individual Filtering

Have ultimate control over your illumination wavelengths with internal paddle-mounted filters with tool-free access.

High Speed

When synchronizing with a camera or other devices, fast turn-on and high speed switching capability is a must. The SPECTRA X® boasts 10µs on time and 5-10 kHz switching rate, enabling high speed applications.

Resources

Datasheet
Type
Document
Manual
Document
Technical Note
Document

pE300whiteFluorescence Light Source

SPECTRA X®

Versatile and Powerful Fluorescence Illumination

Unlock advanced applications in fluorescence with the SPECTRA X®. This 7 channel light engine provides ultimate control over illumination with internal easy-swap excitation filters, individual intensity control over every channel via software, and high-speed TTL interface for synchronizing with other components

  • 7 Channel emission
  • Advanced Control
  • High Speed

Key Features

7 Discrete Spectral Bands

Whatever fluorophore you intend to use, the SPECTRA X® can bring it to life with its broad spectral range.

Advanced Control

Individually control intensities of every illumination channel to fine-tune your experiment. And optional manual control pod makes working with the microscope eyepieces simpler.

High Illumination Power

High maximum illumination power combined with the high-speed switching, this light engine unlocks more demanding fluorescence applications

Individual Filtering

Have ultimate control over your illumination wavelengths with internal paddle-mounted filters with tool-free access.

High Speed

When synchronizing with a camera or other devices, fast turn-on and high speed switching capability is a must. The SPECTRA X® boasts 10µs on time and 5-10 kHz switching rate, enabling high speed applications.

Resources

Datasheet
Type
Document
Manual
Document
Technical Note
Document

MultiCam

SPECTRA X®

Versatile and Powerful Fluorescence Illumination

Unlock advanced applications in fluorescence with the SPECTRA X®. This 7 channel light engine provides ultimate control over illumination with internal easy-swap excitation filters, individual intensity control over every channel via software, and high-speed TTL interface for synchronizing with other components

  • 7 Channel emission
  • Advanced Control
  • High Speed

Key Features

7 Discrete Spectral Bands

Whatever fluorophore you intend to use, the SPECTRA X® can bring it to life with its broad spectral range.

Advanced Control

Individually control intensities of every illumination channel to fine-tune your experiment. And optional manual control pod makes working with the microscope eyepieces simpler.

High Illumination Power

High maximum illumination power combined with the high-speed switching, this light engine unlocks more demanding fluorescence applications

Individual Filtering

Have ultimate control over your illumination wavelengths with internal paddle-mounted filters with tool-free access.

High Speed

When synchronizing with a camera or other devices, fast turn-on and high speed switching capability is a must. The SPECTRA X® boasts 10µs on time and 5-10 kHz switching rate, enabling high speed applications.

Resources

Datasheet
Type
Document
Manual
Document
Technical Note
Document

OptoSplit III

SPECTRA X®

Versatile and Powerful Fluorescence Illumination

Unlock advanced applications in fluorescence with the SPECTRA X®. This 7 channel light engine provides ultimate control over illumination with internal easy-swap excitation filters, individual intensity control over every channel via software, and high-speed TTL interface for synchronizing with other components

  • 7 Channel emission
  • Advanced Control
  • High Speed

Key Features

7 Discrete Spectral Bands

Whatever fluorophore you intend to use, the SPECTRA X® can bring it to life with its broad spectral range.

Advanced Control

Individually control intensities of every illumination channel to fine-tune your experiment. And optional manual control pod makes working with the microscope eyepieces simpler.

High Illumination Power

High maximum illumination power combined with the high-speed switching, this light engine unlocks more demanding fluorescence applications

Individual Filtering

Have ultimate control over your illumination wavelengths with internal paddle-mounted filters with tool-free access.

High Speed

When synchronizing with a camera or other devices, fast turn-on and high speed switching capability is a must. The SPECTRA X® boasts 10µs on time and 5-10 kHz switching rate, enabling high speed applications.

Resources

Datasheet
Type
Document
Manual
Document
Technical Note
Document

OptoSplit II

SPECTRA X®

Versatile and Powerful Fluorescence Illumination

Unlock advanced applications in fluorescence with the SPECTRA X®. This 7 channel light engine provides ultimate control over illumination with internal easy-swap excitation filters, individual intensity control over every channel via software, and high-speed TTL interface for synchronizing with other components

  • 7 Channel emission
  • Advanced Control
  • High Speed

Key Features

7 Discrete Spectral Bands

Whatever fluorophore you intend to use, the SPECTRA X® can bring it to life with its broad spectral range.

Advanced Control

Individually control intensities of every illumination channel to fine-tune your experiment. And optional manual control pod makes working with the microscope eyepieces simpler.

High Illumination Power

High maximum illumination power combined with the high-speed switching, this light engine unlocks more demanding fluorescence applications

Individual Filtering

Have ultimate control over your illumination wavelengths with internal paddle-mounted filters with tool-free access.

High Speed

When synchronizing with a camera or other devices, fast turn-on and high speed switching capability is a must. The SPECTRA X® boasts 10µs on time and 5-10 kHz switching rate, enabling high speed applications.

Resources

Datasheet
Type
Document
Manual
Document
Technical Note
Document

MultiSplit V2

SPECTRA X®

Versatile and Powerful Fluorescence Illumination

Unlock advanced applications in fluorescence with the SPECTRA X®. This 7 channel light engine provides ultimate control over illumination with internal easy-swap excitation filters, individual intensity control over every channel via software, and high-speed TTL interface for synchronizing with other components

  • 7 Channel emission
  • Advanced Control
  • High Speed

Key Features

7 Discrete Spectral Bands

Whatever fluorophore you intend to use, the SPECTRA X® can bring it to life with its broad spectral range.

Advanced Control

Individually control intensities of every illumination channel to fine-tune your experiment. And optional manual control pod makes working with the microscope eyepieces simpler.

High Illumination Power

High maximum illumination power combined with the high-speed switching, this light engine unlocks more demanding fluorescence applications

Individual Filtering

Have ultimate control over your illumination wavelengths with internal paddle-mounted filters with tool-free access.

High Speed

When synchronizing with a camera or other devices, fast turn-on and high speed switching capability is a must. The SPECTRA X® boasts 10µs on time and 5-10 kHz switching rate, enabling high speed applications.

Resources

Datasheet
Type
Document
Manual
Document
Technical Note
Document